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Detection of Hcp, with RNA polymerase (RNAP) as a lysis control. (A) Three isolates showing high hcp transcript levels; (B) 2 isolates showing low hcp transcript levels, (i.e., less than 0.01-fold that of ATCC 19606). Immunoblots show Hcp in whole cells and concentrated culture supernatants prepared from cultures of clinical isolates.
