Table 1.

Ethanol × duration effects on ASPH and related signaling molecules (two-way ANOVA).

Protein	Ethanol factor		Time factor		Ethanol × time
	F-ratio	P-value	F-ratio	P-value	F-ratio	P-value
A85G6-ASPH	30.45	< .0001	207.7	< .0001	3.10	.058
A85E6-ASPH	1.68	N.S.	13.78	< .0001	34.82	< .0001
Notch 1	1.28	N.S.	0.88	N.S.	2.43	.10
Jagged 1	66.79	< .0001	3.11	.05	1.42	N.S.
HES-1	16.30	.0006	9.24	.0004	11.35	.0001
HIF-1α	27.54	< .0001	25.41	< .0001	0.823	N.S.

Frontal lobe white matter homogenates were used in duplex ELISAs with immunoreactivity normalized to RPLPO (see Section 2). Data were analyzed by two-way ANOVA with the Tukey multiple comparison post hoc test. Corresponding data are graphed in Figure 5.