Figure 9.

Annexin (V)expression analysis through flow-cytometer. Expression of Annexin V was evaluated using Annexin V-FITC Apoptosis Detection Kit (Sigma Aldrich, USA). A549 cells infected with A. baumannii strains ATCC 17978, ATCC ΔadeN, adeN complement and PKAB07 were analyzed after incubation for 24 h at 37°C, and statistical significance was derived using 2-tailed Student's t test (*x, P < 0.05; ***, P < 0.001). Experiments were performed in triplicates and error bars represent the standard deviations.