Figure 3.

Protection in murine endotoxemia models. Protective efficacy of mAb A1102 was investigated in the GalN-sensitized murine model of endotoxemia. Groups of 5 mice were passively immunized prophylactically (i.p.) with serial dilutions of A1102 (100 µg to 0.78 µg/mouse doses) or an isotype-matched control antibody (100 µg/mouse). Mice were rendered susceptible to endotoxin by receiving an i.p. injection of GalN 24 h later and at the same time were challenged i.v. with either purified LPS, extracted from a representative ST258 strain, Kp151 (16 ng/mouse) (panel A), or with a minimal lethal dose (1.5–5 × 10⁴ CFU/mouse) of ST258 strains (panel B). Survival was monitored daily for up to 10 d. Graphs represent the combined results from 2 independent experiments for each challenge, with a total group size of 10, except for mice receiving the 0.78 µg dose in live challenges (panel B), which was only included in one of the repeats (n = 5).