Figure 6.

Opsonophagocytotic uptake induced by mAb A1102. RAW264.7 cells were incubated for 30 min at MOI = 1 in the presence of mAb pre-opsonized bacteria and 5% active or heat-inactivated rabbit serum. After the uptake period, gentamicin was added at 100 µg/mL to kill extracellular bacteria. Cells were further incubated for 30 min, and uptake was measured following lysis of cells and plating of surviving intracellular bacteria. Each bar shows the average uptake +/− SEM from 4 individual experiments. Statistical comparison was performed using the Mann-Whitney test.