Figure 5.

Analysis of the influence of truncated rreB 5′-UTRs on EGFP translation. (A) Schematic representation of plasmid pJH-F2 and of its derivatives pJH-14c-egfp, pJH-11c-egfp, and pJH-8c-egfp, which contain rreB-egfp translational fusions. The standard SD and the ATG start codon of pJH-F2 were replaced by the indicated rreB sequences. n.c., cloning of a construct with the full rreB 5′-UTR without the rreB sORF failed. Prrn and Trrn, see Fig. 4A. (B) Western blot analysis with GFP-specific antibodies of strains containing the indicated constructs (see A). EVC, strain containing plasmid pJH-O1. The bottom panel shows a Coomassie Blue stained SDS-polyacrylamide gel after electrophoresis visualizing the loaded protein amounts. Migration of marker proteins (M) in the gel is indicated at the left side in kDa.