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. 2017 Mar 1;14(10):1418–1430. doi: 10.1080/15476286.2017.1297913

Figure 4.

Figure 4.

Recovery of wound healing and S100A11 expression in BC200 RNA-knockdown HeLa cells by BC200 RNA overexpression. (A and B) Wound-healing assays were performed as in Fig. 1 except that HeLa cells were simultaneously transfected with siBC200 I and BC200 expression plasmid DNA. (A) Representative pictures, 40x magnification. (B) Quantitative analyses of wound-healing results. The percentage of recovery was measured and estimated based on the initial wound size of each sample. Shown are Mock-siNegative (red), Mock-siBC200 I (pale red), +BC200-siNegative (blue) and +BC200-siBC200 I (pale blue) (mean ± SD; n = 3; *P < 0.05, by Student's t-test). +BC200, overexpression of BC200 RNA. (C and D) Cells were simultaneously transfected with siBC200 I and BC200 expression plasmid DNA. Total RNA or whole-cell extracts were purified at 24 h post-transfection. (C) Quantitative analysis of S100A11 mRNA and BC200 RNA abundance by qRT-PCR. The abundance of each RNA was normalized to the amount of GAPDH mRNA and depicted as relative RNA levels after dividing with the corresponding RNA abundance in siNegative-treated cells. Shown are Mock-siNegative (red), Mock-siBC200 I (pale red), +BC200-siNegative (blue) and +BC200-siBC200 I (pale blue). (D) Whole-cell extracts were subjected to Western blotting.