Figure 5.

Effects of BC200 RNA knockdown on the transcription of S100A11. (A) Schematic diagrams of S100A11 promoter fragments inserted into a firefly luciferase expression vector pGL3_Basic so as to drive the firefly luciferase reporter gene. The constructs containing the S100A11 promoter regions −2146/+247 and −1712/+247 (+1 at ATG) are p2146 and p1712, respectively. (B) qRT-PCR-based quantification of luciferase transcripts. HeLa cells were serially transfected with the indicated siRNAs, incubated for 24 h, and then transfected with the p2146 or p1712 reporter constructs or with the pGL3_Basic vector (pBasic). At 48 h after the initial siRNA transfection, total RNA was purified and subjected for qRT-PCR (mean ± SD; n = 3). The abundance of firefly luciferase mRNA was normalized to the amount of GAPDH mRNA and depicted as relative RNA levels after dividing with the RNA abundance in siNegative and pGL3_Basic vector-treated cells.