Figure 6.

BC200 RNA knockdown alters the stability of the S100A11 mRNA. (A) HeLa cells were transfected with siRNAs, incubated for 24 h, and treated with actinomycin D (Final concentration: 5 μg/ml). Cells were harvested at 0, 2, 4 and 8 h after the actinomycin D treatment, and total RNA was purified and analyzed by qRT-PCR for S100A11 mRNA. The abundance of S100A11 mRNA was normalized to the amount of GAPDH mRNA in siNegative-, siBC200 I-, or siBC200 II-treated cells, and depicted as relative RNA levels after dividing with the RNA abundance in the corresponding cells before the actinomycin D treatment (mean ± SD; n = 3). (B) The total RNA was also analyzed by semi-qRT-PCR for S100A11 mRNA, BC200 RNA, and 18S rRNA.