Figure 7.

Recovery of the wound healing of BC200 RNA-knockdown HeLa cells by overexpression of S100A11. (A and B) HeLa cells were transfected with siBC200 I or siBC200 II, incubated for 24 h, and then transfected with the S100A11 expression plasmid DNA. Cells were scraped (wounded) at 48 h after the initial knockdown, and the degree of recovery was measured at 0, 12, 24, 36, and 48 h post-wounding. (A) Representative pictures, 40x magnification. (B) The results of our wound-healing assay were quantified as described in the legend to Fig. 1B (mean ± SD; n = 3). Shown are siNegative (red), siBC200 I (blue), and siBC200 II (green). (C) At 48 h after the initial knockdown, whole-cell extracts were prepared and subjected to Western blot analysis.