Figure 8.

Effects of BC200 RNA knockdown on the wound healing of S100A11-knockdown HeLa cells. (A and B) HeLa cells were transfected with siS100A11, incubated for 24 h, and then transfected with siBC200 I or siBC200 II. Cells were scraped (wounded) at 48 h after the initial knockdown, and the degree of recovery was measured at 0, 6, 12, 24, and 36 h post-wounding. (A) Representative pictures, 40x magnification. (B) The results of our wound-healing assay were quantified as described in the legend to Fig. 1B (mean ± SD; n = 3). Shown are siNegative (red), siBC200 I (blue), and siBC200 II (green). (C) At 48 h after the initial knockdown, whole-cell extracts were prepared and subjected to Western blot analysis.