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Principle of dsDNA-recognition by Invader probes (top). Lowest energy structure from MD simulations of 5′-GTGAUATGC:3′-CACTAUACG (bottom left) and 5′-GTGAUATGC:3′-CACTATACG (bottom right) – clearly, the Invader probe is partially unwound and distorted. Lower panel adapted from 24 with permission from the Royal Society of Chemistry.
