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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Org Biomol Chem. 2017 Nov 29;15(46):9760–9774. doi: 10.1039/c7ob02152f

Figure 13.

Figure 13

Detection of chromosomal DNA using Invader probes. Fluorescence micrographs from fluorescence in situ hybridization experiments using Y-chromosome specific Invader probes under non-denaturing conditions. Invader probe 5′-Cy3-AGCCCUGTGCCCTG:3′-TCGGGACACGGGAC-Cy3 was incubated with nuclei from male bovine kidney cells in interphase (upper panel) or metaphase (middle panel), or with nuclei from female bovine fibroblast cells (lower panel). Images viewed using Cy3 (left column) or DAPI (middle column) filter settings; overlays are shown in the right column. Adapted from reference 54 with permission from the Royal Society of Chemistry.