Myogenin abundance and effects of functional overload on plantaris muscle weight. Western blot analysis of myogenin abundance in EDL (A, B) and soleus (C, D) muscle in male (A, C) and female (B, D) DGKζ KO and wild-type mice on chow diet (n = 5–13 mice). Student’s t-test, *P < 0.05 versus wild-type mice. Male DGKζ KO and wild-type mice had 14 days of functional overload on chow diet. Absolute (E) and relative to body weight (F) plantaris muscle weight (n = 6 mice). Two-way ANOVA with Sidak’s post hoc testing. †P < 0.05 surgery effect.