TABLE 4.
LCFA composition of TAGs (LCFATAG) derived from lipin-1-defective patients and normal controls
| LCFATAG C:D | 16:0 (%) | 16:1 (%) | 18:0 (%) | 18:1 (%) | 18:2 (%) |
| LPIN1 def. 1 | 22.82 | 3.82 | 6.37 | 50.03 | 16.96 |
| LPIN1 def. 3 | 25.30 | 4.91 | 7.15 | 49.19 | 13.45 |
| LPIN1 def. 4 | 28.20 | 3.10 | 5.90 | 47.10 | 15.50 |
| Mean patients ± SD | 25.40 ± 2.62 | 4.00 ± 0.84 | 6.46 ± 0.65 | 48.82 ± 1.44 | 15.33 ± 1.76 |
| Control 3 | 33.30 | 4.70 | 4.90 | 46.20 | 10.60 |
| Control 5 | 26.36 | 4.29 | 8.12 | 51.69 | 9.54 |
| Control 6 | 25.71 | 4.92 | 8.41 | 51.53 | 9.43 |
| mean controls ± SD | 29.05 ± 4.28 | 4.86 ± 0.44 | 7.01 ± 1.78 | 48.74 ± 4.06 | 10.34 ± 1.20 |
Total lipid extracts were fractionated using a two-phase extraction method (34). TAGs were separated by TLC as shown in Fig. 3 and LCFATAG analyzed by GC. Data are presented for the different C:D (i.e., for the different lipid number, where C is the number of carbon atoms in the FA and D is the number of double bonds in the FA) as percent (%) of the total of LCFATAG. Mean values ± SD for the LCFATAG (%) of patients and controls are shown for each C:D.