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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Neurotox Res. 2017 Aug 3;32(3):509–517. doi: 10.1007/s12640-017-9788-8

Figure 1. Chronic morphine treatment and morphine withdrawal change the ratio proBDNF/mBDNF in the frontal cortex.

Figure 1

Male rats were treated s.c. with saline or escalating doses of morphine for five days and were euthanized 2 hr later. Another group of rats received an acute dose of morphine 2 hr prior to euthanasia. A third group of animals received chronic morphine and was allowed to undergo spontaneous withdrawal for 60 hr. The levels of mBDNF and proBDNF were then measured by Western blot in tissue lysates from the frontal cortex following immunoprecipitation. A: Example of Western blot analysis. Lysates were immunoprecipitated with an antibody against BDNF. The blot was then analyzed with a BDNF antibody that recognizes both the mature (14kDa) and pro form of BDNF (34 kDa). B. Densitometric analysis of BDNF-like immunoreactivity expressed as the ratio mBDNF/proBDNF immunoreactivity. Data are the mean ± SEM of 4 rats per group. *p<0.01 vs saline, #p<0.05 vs chronic morphine.