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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Neurotox Res. 2017 Aug 3;32(3):509–517. doi: 10.1007/s12640-017-9788-8

Figure 5. Morphine increases the release of mBDNF and proBDNF in vitro.

Figure 5

Rat cortical neurons were exposed to 1 μM, morphine or saline control each day for five days. (A) Medium was collected at the indicated time points, and levels of BDNF and proBDNF were measured by ELISA in the concentrated media. Data are expressed as % of control because the basal levels of neurotrophins fluctuated throughout the experiments. (B) Cortical neurons were exposed to morphine or saline control for 3 days. Starting at day 3, PAI-1 (100ng/ml) was added concomitantly with morphine. Data, expressed as % of control, are the mean ± SEM of three separate experiments in duplicate. *p<0.01, #p<0.05 vs control.