Table 1.

Concordance between mRNA quantification and IHC/FISH.

	IHC/FISH positive*	IHC/FISH negative	total
dCt ≥−1	26	3	29
dCt < −1	4	47	51
total	30	50	80

^*IHC/FISH defined as positive if clinically IHC =3+ or IHC 2+ and FISH amplified. IHC/FISH defined as negative if clinically IHC=0 or 1+ or IHC=2+ but FISH not amplified using ASCO/CAP 2007 guidelines.

Abbreviations: IHC = Immunohistochemistry, FISH= Fluorescence In Situ Hybridization, dCt = delta ct or difference in cycle threshold (see methods section)