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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Oct 23;41(12):2051–2065. doi: 10.1111/acer.13499

Figure 2.

Figure 2

SMC α-actin expression in Sca1-eGFP transgenic mice carotid cross sections from sham-operated (Sham), ligated (cornstarch), and ligated treated with Ethanol (Ligated + EtOH) groups. Immunohistochemistry for smooth muscle specific α-actin was performed on sections from vessels harvested 14 days post ligation or sham-operation, using an anti alpha-smooth muscle actin (α-SMA) antibody (Abcam ab5694). Representative images shown; blue = Dapi nuclear stain, Green = eGFP (i.e., Sca1+), red = α-actin. ×20 magnification on left (scale bars 50 μM); ×60, of boxed portion, shown on right. Width of Adventitia ‘A’ denoted by yellow line, Media: ‘M’ white line, Neo-intima: ‘NI’ red line.