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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Oct 23;41(12):2051–2065. doi: 10.1111/acer.13499

Figure 4. EtOH inhibits Patched1 expression in ligated carotids.

Figure 4

The protein expression of Sonic hedgehog target gene Patched 1 (Ptch1) in carotid cross sections from the different experimental groups [sham-operated, ligated (cornstarch control), ligated + EtOH] was assessed by immunohistochemistry using a rabbit polyclonal anti-Ptch1 primary antibody and an Alexa fluor 594-conjugated secondary antibody. Representative images (×20 magnification, scale bar 50 μM) shown. DAPI (blue); Ptch1 (red), eGFP (green, i.e., Sca1+), merged image (bottom). Dotted lines trace internal and external elastic laminae.