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. Author manuscript; available in PMC: 2018 Dec 29.
Published in final edited form as: J Chromatogr A. 2017 Nov 14;1530:112–119. doi: 10.1016/j.chroma.2017.11.023

Figure 2.

Figure 2

Retention factor of 4-methylhippuric acid on BEH-C18 (red) and Hypercarb (black) columns as a function of methanol volume fraction in the mobile phase. Columns were a 2.1 mm × 50 mm packed with 1.7 μm BEH-C18 and a 4.6 mm × 100 mm packed with 3 μm Hypercarb. Mobile phases consisted of mixtures of water/MeOH + 0.1% FA at 30 °C. Experimental data fit with exponential curves.