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. Author manuscript; available in PMC: 2017 Dec 28.
Published in final edited form as: J Am Chem Soc. 2016 Dec 16;138(51):16584–16587. doi: 10.1021/jacs.6b09682

Table 1.

Physical, Spectroscopic, and Magnetic Field-Sensing Properties of the Single-Trp Series of Flavomaquettes

design Tm
(°C)		 ΦFa τF
(ns)b 		Trp Fluor. max.
(nm)		 Flavin Emc
(mV vs SHE)		 Trp Emc
(V vs SHE)		 Predicted ET rate
(s−1)d 		MFE (%, 520–610 nm)
control 49.0 0.11 1.150 – −110 – – 0
W13 50.5 0.04 0.606 335 −137 1.10 2.55 × 1011 +16.0
W16 53.2 0.07 0.846 338 −128 1.08 1.11 × 108 +17.5
W20 55.3 0.10 0.974 337 −130 1.06 4.86 × 104 0
a

Quantum yield of flavin fluorescence.

b

Amplitude-averaged lifetime of flavin fluorescence obtained from a triexponential fit to TCSPC data.

c

Midpoint potentials.

d

Rate constants for forward electron transfer to the triplet state flavin predicted using the Moser–Dutton ruler.12