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. 2017 Nov 27;4:77. doi: 10.3389/fmolb.2017.00077

Figure 4.

Figure 4

Effect of Lys13 acetylation on protein-DNA interaction. (A) Electrophoretic mobility shift assay. BamHI-treated linearized plasmid DNA, pBK AcKRS, (250 ng) was incubated with different amount proteins as indicated in binding buffer (30 mM Tris pH 7.5, 100 mM NaCl, 0.02% v/v Tween20, 0.5 mg/ml BSA) at 37°C for 1 h before electrophoresed on a 1% agarose gel. Electrophoretic mobility shift assay results under other conditions are shown in Figure S6. (B–D) Biolayer interferometry analysis of wt* (B), wt (C), and K13ac (D) HU proteins. A representative sensorgram from triplicates is displayed here for each protein at 480, 192, 76.8, 30.7, and 12.3 nM. Asterisk (*) denotes protein without His-tag. The exact amino acid sequence of each protein construct is shown in Figure S4.