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. 2017 Nov 27;11:110. doi: 10.3389/fnana.2017.00110

Figure 3.

Figure 3

Graphs showing the average somal area (A,C,E) and average somal volume (B,D,F) of cholinergic (ChAT; A,B), catecholaminergic (TH; C,D) and orexinergic (OxA; E,F) immunoreactive cells in the brains of three different groups (n = 6 per group) analyzed in the present study, the group exposed to chronic prenatal alcohol (CA), the prenatal gavage control group (CAc) and the NTc. The average somal area and volume of the ChAT+ neurons of the LDT and PPT nuclei (A,B) in the CA group are statistically significantly (*) smaller than the control groups (CAc: parea = 4 × 10−5; pvolume = 4 × 10−5 respectively and NTc: parea = 0.017; pvolume = 0.070). The average somal area and volume of the TH+ neurons of the LC (C,D) are not statistically significantly different between the three groups. The average somal area and volume of the OxA+ neurons of the hypothalamus (E,F) in the CA group are statistically significantly (*) larger than the control groups (CAc: parea = 0.026; pvolume = 0.030) and NTc: parea = 5 × 10−5; pvolume = 9 × 10−5).