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. 2017 Dec 1;7:16800. doi: 10.1038/s41598-017-17118-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colonies of Mucor circinelloides carB disruption mutants obtained by the CRISPR-Cas9 method. Panel A, selection of the transformed colonies obtained from the wild-type strain, CBS227.49 using NHEJ mutagenesis based on their white colony color. Panel B, colony color of the strain CBS227.49 and its carB′ disruption mutants (CBS227.49-carB′/1 and CBS227.49-carB′/2) created by NHEJ mutagenesis. Panel C, colony color of the strain MS12 and its carB disruption mutants created by NHEJ (MS12-carB′/1 and MS12-carB′/2) and HDR (MS12-carB + pyrG/1 and MS12-carB + pyrG/2) approaches.