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. 2017 Dec 1;7:16800. doi: 10.1038/s41598-017-17118-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome editing strategy designed to disrupt the carB gene of Mucor circinelloides by the CRISPR-Cas9 method and positions of the primers used in the study (A) and PCR analysis of the transformants using the MccarB1 and MccarB2 primers (Table 2) (B). TGG indicates the PAM sequence; arrows show the orientations of the primers. Panel B: M, GeneRuler 1 kb DNA ruler (Thermo Scientific); 1, MS12 strain; 2, MS12-carB + pyrG/1 strain; 3, MS12- carB + pyrG/2 strain.