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. 2017 Dec 1;7:16757. doi: 10.1038/s41598-017-16727-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of local sustained release of NGF (10 ng/ml) from hydrogels placed in front of the attached OVs. Local sustained release of NGF using hydrogel promoted axonal growth, stained by NFL in hESC- and hiPSC-derived RGCs ((b) and (f), respectively, magnification 40x), as compared with controls ((a) and (e), respectively). The shape of the filopodia of hESC- and hiPSC-derived RGCs ((d) and (h), respectively) was slightly promoted by NGF release, as compared with controls ((c) and (g), respectively), as evident from the staining of phalloidin and GAP43. The assessment from D24-D31 is performed in RMM. Each experiment was repeated at least five times. Scale bars in (a), (b), (e) and (f), 100 μm. Scale bars (c), (d), (g) and (h), 10 μm.