Table 1.
Gene expressions with significant differences among groups in adults.
| Brain region | Genes | F-statistics, degree of freedom, and p-values for two-way ANOVA | Expression levels | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| For each factor and interaction | Fold differences | Male | Female | ||||||||
| Genotype | Sex | Genotype × Sex | WT vs. Crmp4-KO (Crmp4-KO/WT) | Male vs. Female (Female/Male) | WT | Crmp4-KO | WT | Crmp4-KO | |||
| Olfactory bulb | GluR2 | F(3,12) = 3.013, p = 0.072 | F(1,12) = 5.843, p = 0.032 | F(1,12) = 1.215, p = 0.292 | F(1,12) = 1.981, p = 0.185 | 1.969 | 1.352 | 0.532 ± 0.188 | 0.564 ± 0.141 | 0.472 ± 0.122 | 0.952 ± 0.120* |
| VGluT1 | F(3,12) = 16.871, p < 0.001 | F(1,12) = 36.167, p < 0.001 | F(1,12) = 11.327, p = 0.006 | F(1,12) = 3.117, p = 0.103 | 1.389 | 0.833 | 0.815 ± 0.025† | 1.009 ± 0.062* | 0.585 ± 0.028 | 0.935 ± 0.053* | |
| VGluT2 | F(3,12) = 3.191, p = 0.063 | F(1,12) = 1.300, p = 0.276 | F(1,12) = 5.707, p = 0.034 | F(1,12) = 2.566, p = 0.135 | 1.116 | 1.266 | 0.849 ± 0.051 | 0.803 ± 0.029† | 0.920 ± 0.170 | 1.172 ± 0.032 | |
| GABAAα1 | F(3,12) = 62.224, p < 0.001 | F(1,12) = 78.549, p < 0.001 | F(1,12) = 45.705, p < 0.001 | F(1,12) = 62.418, p < 0.001 | 1.642 | 1.455 | 1.367 ± 0.120 | 1.459 ± 0.059† | 1.259 ± 0.051 | 2.854 ± 0.126* | |
| GABAAγ2 | F(3,12) = 11.147, p = 0.001 | F(1,12) = 20.106, p = 0.001 | F(1,12) = 1.439, p = 0.253 | F(1,12) = 11.894, p = 0.005 | 1.524 | 1.118 | 1.274 ± 0.112 | 1.410 ± 0.194† | 0.978 ± 0.059 | 2.021 ± 0.126* | |
| GABABR1 | F(3,12) = 7.722, p = 0.044 | F(1,12) = 16.841, p = 0.001 | F(1,12) = 4.138, p = 0.065 | F(1,12) = 2.184, p = 0.165 | 1.839 | 1.342 | 0.182 ± 0.010 | 0.285 ± 0.068† | 0.204 ± 0.036 | 0.424 ± 0.012* | |
| VGAT | F(3,12) = 7.897, p = 0.004 | F(1,12) = 12.939, p = 0.004 | F(1,12) = 1.182, p = 0.298 | F(1,12) = 9.570, p = 0.009 | 1.426 | 1.113 | 0.317 ± 0.029 | 0.335 ± 0.057† | 0.249 ± 0.015 | 0.476 ± 0.018* | |
| Ncam1 | F(3,12) = 7.576, p = 0.004 | F(1,12) = 20.543, p = 0.001 | F(1,12) = 2.124, p = 0.166 | F(1,12) = 0.012, p = 0.913 | 1.839 | 1.342 | 1.214 ± 0.043 | 1.573 ± 0.051* | 1.085 ± 0.096 | 1.462 ± 0.113* | |
| N-cadherin | F(3,12) = 2.283, p = 0.131 | F(1,12) = 6.782, p = 0.023 | F(1,12) = 0.007, p = 0.936 | F(1,12) = 0.061, p = 0.809 | 1.321 | 0.913 | 1.178 ± 0.067 | 1.385 ± 0.041 | 1.149 ± 0.153 | 1.400 ± 0.035 | |
| Hippocampus | GluR1 | F(3,12) = 3.793, p = 0.040 | F(1,12) = 1.031, p = 0.330 | F(1,12) = 0.916, p = 0.357 | F(1,12) = 9.431, p = 0.010 | 1.171 | 0.861 | 0.544 ± 0.066 | 0.999 ± 0.121*† | 0.860 ± 0.164 | 0.550 ± 0.114 |
| GluR2 | F(3,12) = 2.857, p = 0.086 | F(1,12) = 6.301, p = 0.029 | F(1,12) = 0.106, p = 0.750 | F(1,12) = 2.874, p = 0.118 | 1.505 | 1.055 | 0.227 ± 0.061 | 0.469 ± 0.044* | 0.343 ± 0.022 | 0.400 ± 0.101 | |
| VGluT1 | F(3,12) = 1.942, p = 0.177 | F(1,12) = 5.266, p = 0.041 | F(1,12) = 0.169, p = 0.666 | F(1,12) = 0.365, p = 0.557 | 1.160 | 0.973 | 0.916 ± 0.079 | 1.017 ± 0.042 | 0.850 ± 0.073 | 1.031 ± 0.047 | |
| GABAAα1 | F(3,12) = 3.623, p = 0.045 | F(1,12) = 3.983, p = 0.069 | F(1,12) = 5.771, p = 0.033 | F(1,12) = 1.116, p = 0.312 | 1.211 | 1.265 | 0.194 ± 0.010 | 0.215 ± 0.010† | 0.224 ± 0.010 | 0.291 ± 0.041 | |
| GABABR1 | F(3,12) = 3.548, p = 0.048 | F(1,12) = 0.379, p = 0.549 | F(1,12) = 10.185, p = 0.008 | F(1,12) = 0.078, p = 0.784 | 1.071 | 1.514 | 0.135 ± 0.008 | 0.144 ± 0.008† | 0.204 ± 0.034 | 0.220 ± 0.027 | |
| N-cadherin | F(3,12) = 2.266, p = 0.133 | F(1,12) = 5.333, p = 0.040 | F(1,12) = 1.074, p = 0.321 | F(1,12) = 0.392, p = 0.543 | 0.948 | 1.126 | 1.028 ± 0.046 | 0.939 ± 0.037 | 1.118 ± 0.032 | 1.096 ± 0.083 | |
| Cortex | VGluT2 | F(3,12) = 3.920, p = 0.037 | F(1,12) = 5.273, p = 0.040 | F(1,12) = 6.351, p = 0.027 | F(1,12) = 0.135, p = 0.719 | 0.735 | 0.712 | 0.136 ± 0.021 | 0.100 ± 0.009 | 0.097 ± 0.012 | 0.071 ± 0.007 |
| GABAAγ2 | F(3,12) = 5.557, p = 0.013 | F(1,12) = 14.229, p = 0.003 | F(1,12) = 0.018, p = 0.896 | F(1,12) = 2.425, p = 0.145 | 0.667 | 1.011 | 0.097 ± 0.013 | 0.077 ± 0.006 | 0.113 ± 0.011 | 0.064 ± 0.004* | |
| GABABR1 | F(3,12) = 5.623, p = 0.012 | F(1,12) = 7.181, p = 0.020 | F(1,12) = 7.752, p = 0.017 | F(1,12) = 1.937, p = 0.189 | 0.773 | 0.765 | 0.115 ± 0.012† | 0.081 ± 0.008* | 0.080 ± 0.006 | 0.069 ± 0.007 | |
| Ncam1 | F(3,12) = 9.911, p = 0.001 | F(1,12) = 29.650, p < 0.001 | F(1,12) = 0.011, p = 0.919 | F(1,12) = 0.071, p = 0.795 | 0.671 | 1.008 | 1.227 ± 0.085 | 0.839 ± 0.068* | 1.255 ± 0.084 | 0.827 ± 0.060* | |
| N-cadherin | F(3,12) = 20.827, p < 0.001 | F(1,12) = 6.446, p = 0.026 | F(1,12) = 53.076, p < 0.001 | F(1,12) = 2.960, p = 0.111 | 0.484 | 1.276 | 1.105 ± 0.121 | 0.592 ± 0.029* | 1.498 ± 0.117 | 0.667 ± 0.070* | |
Asterisks indicate significant differences between WT and Crmp4-KO mice of same sex (*p < 0.05, two-way ANOVA followed by Fisher’s PLSD post-hoc tests). Dagger indicates significant difference between male and female mice of the same genotype († p < 0.05, two-way ANOVA followed by Fisher’s PLSD post-hoc tests). Data are expressed as mean ± SEM.