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. 2017 Nov 27;8:1649. doi: 10.3389/fimmu.2017.01649

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Copyright © 2017 Wu, Lu, Zhang, Mei, Feng, Peng, Hou, Kang, Liu and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hemagglutinin inhibition (HI) cross-reactivity of H5 rHA protein to sera raised by different vaccines. The HI tests were carried out with the recombinant protein of consensus H5 HA (rHA), viral antigens of Re5, Re6, Re7, and Re8 as 4 hemagglutinin units (HAU)/25 μl in three replicates. The six inner genes of Re5, Re6, Re7, and Re8 vaccine strains are derived from A/Puerto Rico/8/1934 virus (PR8, H1N1), the HA and neuraminidase genes of Re5 from strain of A/duck/Anhui/1/2006 (clade 2.3.4), of Re6 from virus of A/duck/Guangdong/S1322/2006 (clade 2.3.2), of Re7 from A/Chicken/Liaoning/S4092/2011 (clade 7.2), and of Re8 from A/chicken/Guizhou/4/13 (clade 2.3.4.4). The statistical significance was analyzed with Student’s t-test. ***P < 0.001.