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. 2017 Nov 27;8:189. doi: 10.3389/fgene.2017.00189

Figure 4.

Figure 4

Luciferase assay results. (A) HEK293T transfection, GFP signal showed that transfection rate was about 70% and pEGFP-C1 vectors were functional. (B) Relative luciferase activity was decreased in wild type 3′-UTR (WT-UTR) (P = 0.0002) after miR-191 overexpression, but there was no significant alteration in relative luciferase activity when miR-191 target site was deleted from 3′-UTR (MT-UTR) (P = 0.1019). (C) miR-222 significantly decreased relative luciferase activity in wild type 3′-UTR (WT-UTR) condition (P = 0.0020), but there was no significant change in relative luciferase activity when miR-222 target site was omitted from the 3′-UTR (MT-UTR) (P = 0.1238). (D) miR-224 overexpression effect on relative luciferase activity in wild type 3′-UTR (WT-UTR) and mutated 3′-UTR (MT-UTR) conditions. miR-224 significantly decreased relative luciferase activity in WT-UTR (P = 0.0071), but there was no significant change in relative luciferase activity when miR-224 target site was deleted (MT-UTR) (P = 0.4906).