Figure 1.
Expression and frequency of TIM3+ MDM is not modified when monocytes are exposed to LAM. Pure monocytes were exposed to LAM for 1, 2 3, 4, or 5 days and at day 7 cells were recovered to perform flow cytometry or qPCR. Flow cytometry representative zebra plot of MDM without stimulus or exposed to LAM for 4 days (left and right, respectively) (A). Expression of TIM3 was analyzed by flow cytometry independent or dependent of CD14 co-expression (B and C). TIM3 relative gene expression was measured by real-time PCR (D). Soluble form of TIM3 was measured in supernatant by ELISA (E). Data are representative of five independent experiments. Box plot indicates median ± IQR (5-95). Kruskal-Wallis and Dunn post-hoc tests compared to unexposed MDM.