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. 2017 Nov 27;8:868. doi: 10.3389/fphar.2017.00868

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Copyright © 2017 Sharath Babu, Anand, Ilaiyaraja, Khanum and Gopalan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nrf2 nuclear localization in HepG2 cells was examined by confocal microscopy after pretreatment with PC for 2 h followed by with or without CTN (96 μM) treatment for 24 h (a) Control, (b) CTN 96 μM, (c) PC 100 μM, (d) PC 50 μM + CTN 96 μM, (e) PC 100 μM + CTN 96 μM. Green signals represent nuclear distribution of Nrf2 while nuclei were stained with Hoechst 33342 (blue signal) as assessed by secondary antibody conjugated with FITC staining in laser scanning confocal microscope. Scale bar, 10 μm.