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. 2017 Nov 27;8:868. doi: 10.3389/fphar.2017.00868

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Copyright © 2017 Sharath Babu, Anand, Ilaiyaraja, Khanum and Gopalan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photomicrographs of HepG2 cells stained with acridine orange (AO, green) and ethidium bromide (EtBr, red) fluorescent dyes, (A) Vital cells (green), early apoptotic (bright green to yellow), late apoptotic (orange) and necrotic cells (red). (a) Control, (b–e) CTN 25, 50, 75, and 100 μM, (f) Control, (g) CTN 96 μM, (h) PC 100 μM, (i) PC 50 μM + CTN 96 μM, (j) PC 100 μM + CTN 96 μM. (B) The percentage of apoptotic and necrotic cells after staining with AO and EtBr. Data are expressed as the mean ± SD. ^∗p < 0.05 vs. control cells, ^#p < 0.05 vs. CTN treated cells. Scale bar, 20 μm.