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. 2017 Dec 1;7:16784. doi: 10.1038/s41598-017-17021-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Variable Lck expression levels in CLL cells determined by Western blot and flow cytometry. (a) Western blot analysis of Lck expression in lysates of purified CLL cells. 10 μg of protein derived from lysates of CD19-purified CLL cells was separated by SDS-PAGE and immunoblots probed with either anti-Lck mAbs or anti-β-actin (as loading control). Varying amounts of purified recombinant Lck protein were used as standards to determine Lck levels in cell lysates. (b) Flow cytometry histograms of Lck expression in CLL cells derived from cases with known high and low levels. (c) Correlation of Lck expression in CLL cells determined by Western blot versus flow cytometry.