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. 2017 Dec 1;7:16753. doi: 10.1038/s41598-017-17074-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Region of interest anatomical definition: (A) Human cadaver brainstem specimen used during a neuro-pathological dissection. A consultant neuropathologist dissected the brainstem specimen and identified the anatomical landmarks of the left and right superior colliculi. The diameter of each superior colliculus was measured and noted to be 7 mm. (B) Superior collicular 3-D regions of interest on T1-weighted structural brain images. Three radiological views are shown; axial, coronal and sagittal respectively. Mango image processing software (Lancaster, Martinez; www.ric.uthscsa.edu/mango) was used to create the 3-D regions of interest using pre-defined anatomical boundaries from the neuro-pathological dissection.