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. 2017 Dec 1;7:16813. doi: 10.1038/s41598-017-16874-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chitosan causes auxin accumulation in Arabidopsis roots. (A) Two hours exposition to liquid chitosan provoke changes in DR5:GUS localisation in primary roots exposed this polymer. Untreated plants shows DR5:GUS expression located in the meristematic zone and stem cell niche. Plants exposed to chitosan showed accumulation of DR5:GUS expression in the transition zone and meristematic zone. (Scale bar 50 µm). (B) Chitosan causes mislocalisation of DR5rev:GFP in secondary roots. DR5rev:GFP localise in the meristematic zone and vascular tissue. Arrows indicate expression of DR5 in the meristematic zone. (Scale bar 50 µm).