Fig. 2.

ILC2₁₀ constitute a major subset of IL-10 producing lung hematopoietic cells. a, b Flow cytometry analysis of ILC2 (a) or T_reg cells (b) from the lungs of 10BiTFoxp3 ^GFP dual reporter mice treated with PBS (left) or IL-33 (right). c, d Quantitation of the frequency (c) and number (d) of lung ILC2₁₀ and T_reg cells, calculated from gates as in a and b, here and in all subsequent figures. e Il10 gene expression from total ILC2 from wild-type mice (red) or subsets of ILC2 from 10BiTFoxp3 ^GFP mice (pink) treated with PBS or IL-33. f Frequency of cell types including ILC2 as in a, T_reg cells as in b, non-T_reg T cells (Lin⁺Thy-1.2⁺GFP^–) or other cells (Lin⁺Thy-1.2^–GFP^–), among the total CD45⁺IL-10⁺ population from the lungs of 10BiTFoxp3 ^GFP animals treated with PBS or IL-33. g Analysis of lung ILC2₁₀ in 10BiTFoxp3 ^GFP animals following chronic treatment with PBS or papain. h Compiled data of frequency of ILC2₁₀ in the lungs of papain-treated 10BiTFoxp3 ^GFP mice gated as in a. Data are from at least two independent experiments with representative plots shown in a, b, f, g and compiled data on individual mice shown in c, d, e, h. ***P < 0.001, **P < 0.01, *P < 0.05, and ns (not significant, P ≥ 0.05) (Student’s t test)