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. 2017 Dec 1;7:16756. doi: 10.1038/s41598-017-17006-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression analysis of Medicago cell lines expressing L-PGDS, with and without addition of 2.5 µM SAHA (at days 3 or 7 of growth). The calculated sample quantity (Copies/µL) represents the concentration of the cDNA sample in the PCR reaction mix. ***p value ≤ 0.001. Error bars indicate the theoretical confidence interval based on Poisson distribution calculated by the QS3D Analysis Suite^TM software for n = 6 (control) and n = 3 (SAHA).