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. 2017 Dec 1;7:16736. doi: 10.1038/s41598-017-16930-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM analysis of anthers from wild-type and ms6021. (A) to (D) Microspore (A,B) and tapetum (C,D) of anthers from wild type (A,C) and the mutant (B,D) during the uninucleate stage. (E) to (H) Microspore (E,F) and pollen exine (G,H) of anther from wild-type (E,G) and mutant (F,H) at large vacuole stage. (I) to (L) Microspore (I,J) and pollen exine (K,L) of anthers from wild type (I,K) and the mutant (J,L) at the binucleate stage. (M) to (P) Anther wall (M,N) and pollen exine (O,P) of anthers from wild type (M,O) and the mutant (N,P) at the mature pollen stage. (Q) to (T) Anther epidermal surface of wild type (Q,S) and the ms6021 mutant (R,T) at the uninucleate stage (Q,R) and mature pollen stage (S,T). C, cavity for dehiscence. Cu, cuticle; CW, cell wall; ER, endoplasmic reticulum; Ex, exine; In, intine; LL, lipidosome-like; Msp, microspore; N, nucleus; T tapetum; Ub, ubisch body; V, vacuole. G and H were zoomed form black solid boxes region in E and F respectively. Bars = 5 µm in (A,B,E,F,I,J,M and N), 2 µm in (C,D), and 0.5 µm in (G,H,K,L,O and P) and (Q) to (T).