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. 2017 Dec 1;7:16736. doi: 10.1038/s41598-017-16930-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular cloning and sequence analysis of MS6021. (A) Fine mapping of the MS6021 on chromosome 9. The location and name of the makers are indicated. The MS6021 locus was mapped to a 15-kb region between markers 4–49 and 4–89. (B) A schematic representation of the exon and intron structure of MS6021. The black boxes indicate exons, grey boxes indicate UTR region, and intervening lines indicate introns. +1 indicates the start codon (ATG); +1782 indicates the stop codon (TAA). A 926-bp fragment is inserted into the 3′UTR in ms6021 and ms6022; 2-bp is inserted into the third exon in ms6046; a 2142-bp fragment from the first intron to the last exon is deleted in ms6047. (C) The amino acid sequence of MS6021. The grey and black lines underlining indicated putative NAD-binding region and active region respectively.