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. 2017 Dec 1;7:16736. doi: 10.1038/s41598-017-16930-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern of MS6021. (A) qRT- PCR analysis of MS6021 expression. Error bars indicate the SD (n = 3). (B) Protein gel blot analysis. Full-length gels are presented in Supplementary Figure S4. Te, tetrad stage; Un-a, early uninucleate stage; Un-b, late uninucleate stage. (C) to (F) RNA in situ hybridization using an anti-sense probe during the tetrad stage (C), early uninucleate stage (D), large vacuole stage (E), and binucleate stage (F). (G) RNA in situ hybridization using a sense probe during the early uninucleate stage. Msp, microspore; T, tapetum. Bars = 50 µm in (C) to (F) and 100 µm in (G).