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. 2017 Dec 1;7:16736. doi: 10.1038/s41598-017-16930-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional conservation of MS6021. (A) A neighbor-joining phylogenetic tree summarizing the evolutionary relationships among FAR members in Arabidopsis, rice, and maize of (BLASTPE E-value < 1E-100). The proteins are named according to their Phytozome accession numbers. The numbers under the branches refer to the bootstrap value of the neighbor-joining phylogenetic tree. The length of the branches is proportional to the amino acid variation rates. At, Arabidopsis thaliana; Os, Oryza sativa; Zm, Zea mays. The bar indicates the estimated number of amino acid substitutions per site (for the protein alignment, see Figure S4). (B) to (E) SEM analysis of the pollen wall surface of wild-type (B), ms2 mutant (C), and transgenic ms2 mutant lines (D and E) at anthesis. Bars = 5 µm.