Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 1;7:16741. doi: 10.1038/s41598-017-16842-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Single molecule fluorescence measurements reveal that purified WT and Quad opsins are monomers. (A) Schematic illustration of the single molecule pulldown (SiMPull) set up. (B) Representative TIRF image of SNAP-surface 549-labeled WT opsin-FLAG-SNAP; the circled spot corresponds to the photobleaching trace in panel D. (C) Representative TIRF image of SNAP-surface 549-labeled QUAD opsin-FLAG-SNAP; the circled spot corresponds to the photobleaching trace in panel E. (D) Trace depicting 1-step photobleaching of the circled spot from panel B when exposed to a 561-nm laser beam at time 0 s. The arrow depicts the point at which photobleaching occurred. (E) Trace depicting 1-step photobleaching of the circled spot from panel C when exposed to a 561-nm laser beam at time 0 s. The arrow depicts the point at which photobleaching occurred. (F) Fraction of the total population of spots that show 1-, 2-, 3-, or 4-step photobleaching (1102 and 1083 spots were analyzed for WT and QUAD opsin constructs, respectively). Error bars indicate standard errors calculated from 5 movies for each condition (169–257 spots were analyzed per movie).