Table 1.

Fit parameters for fluorescence reduction traces.

	n	Half-life (sec)	Magnitude of line slope S (sec−1 × 104)
No protein	6	20.23 ± 1.79	1.20 ± 0.22
WT	20	22.63 ± 1.26	1.40 ± 0.16
QUAD	15	23.37 ± 0.87	1.40 ± 0.13

Fluorescence traces from scramblase assays performed over a range of PPR values, including those shown in Fig. 5, were fit to the equation F(t) = (1 − Plateau)^*exp(−K^*t) + Plateau − S^*t, where F(t) = fluorescence at time t, t = 0 sec is the time of dithionite addition, and S = absolute value of the slope of the linear component. The results are provided as mean ± SEM (n = number of independent vesicle reconstitution samples). The standard error of individual fits was at least an order of magnitude lower than the SEM. Values of half-life (=0.69/K) and S are provided. The similar half-life values obtained irrespective of the PPR of the vesicles confirms that the dithionite reaction is rate-limiting in all cases. Only the plateau values (not shown here) vary with PPR – these are used to calculate the data fits described in Table 2.