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. 2017 Dec 1;7:16713. doi: 10.1038/s41598-017-16308-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Off-target sites and T7 endonuclease cleavage assay of potential off-target loci. (a) Ranked list of potential off-target loci for R124H mutation-specific gRNA. Number of mismatched bases, PAM sequence, chromosomes, and target gene are indicated. (b) Sequence alignments of R124H mutation-specific gRNA and potential off-target loci. (c) Potential off-target sites in edited cells were amplified by PCR. After T7 endonuclease treatment, no off-targets effects were found at any site. N/A: not applicable.