Table 2.
Mutations identified in the evolved strains UBw and UBm.
| Gene/Locus | Annotation | Mutation | UBw Freq## | Ubm Freq## |
|---|---|---|---|---|
| corA, cg0080 | Putative CorA-like Mg2+/Co2+ transporter protein, MIT-family | Insertion of tnp13b (cg1782) | 0.78## | 0 |
| corA, cg0080 | Putative CorA-like Mg2+/Co2+ transporter protein, MIT-family | Deletion 12 bp, CGTCGACGATGG, position 593 to 604# | 0 | 68/69 |
| hmuV, cg0469 | hemin transport system, ATP-binding protein | Exchange S73Y | 5/46 | 0 |
| whmD, cg0850 | whmD homolog | G to A mutation, 181 bp upstream# | 0 | 166/166 |
| -, cg0934 | hypothetical protein, conserved | Deletion 1 bp, T at position 1456# | 0 | 151/160 |
| Intergenic, cg1696 and aspA, cg1697 | cg1696, permease of the major facilitator superfamily and aspA, aspartate ammonia-lyase (aspartase) (EC 4.3.1.1) | Insertion of tnp13b (cg1782) in IGR | 0 | 0.60 |
| psp1, cg2069 | cg2069, psp1, putative secreted protein, CGP3 region | Exchange V123L | 125/125 | 0 |
| soxA, cg1781 | soxA, sarcosine oxidase- C-terminal fragment | Exchange L26L, codon exchange TTA to CTA | 83/207 | |
| fruK, cg2119 | 1-phosphofructokinase (EC:2.7.1.56) | Exchange R71L | 0 | 168/168 |
| pyk, cg2291 | pyruvate kinase (EC:2.7.1.40) | Exchange A20V | 60/60 | 0 |
| pyk, cg2291 | pyruvate kinase (EC:2.7.1.40) | Exchange A271T | 0 | 171/171 |
| -, cg2380 | putative membrane protein | Deletion 1 bp, C, position 270# | 121/124 | 0 |
| -, cg2380 | putative membrane protein | Stop Q93* | 0 | 159/159 |
| psp5, cg3197 | putative secreted protein | S270S | 142/142 | 0 |
| cg3226 | lactate permease | Insertion of tpn5a (cg0824) | 0.91## | 0 |
#Position 1 is the first bp of the start codon from the respective gene.
##Frequencies of SNVs (or MNVs) are given by the count/coverage ratio and transposon insertions are described by the ratio to the variant sequence.