Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 1;7:16802. doi: 10.1038/s41598-017-17103-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of leptin on Akt and MEK pathways and impact of inhibition of Akt and MEK pathways on profibrotic protein factors and ROS levels in cardiac myofibroblasts. Protein levels of (a) pAkt/Akt and (b) pERK 1/2/ERK1/2 stimulated by leptin (100 ng/mL) for indicated time intervals. Cardiac myofibroblasts stimulated for 24 hours with leptin (100 ng/mL) in the presence or absence of the inhibitors of either MEK (PD98059; PD; 25 × 10⁻⁶ mol/L) or Akt (LY294002; LY; 20 × 10⁻⁶ mol/L) pathways for 24 hours. (c) Protein levels of collagen type I, TGF-β, CTGF and galectin-3. (d) Representative microphotographs in cells labeled with DHE and (e) Quantification of total superoxide anions and. Bar graphs represent the mean ± SD of 3–4 assays in arbitrary units normalized to β-actin. *p < 0.05; ***p < 0.001 vs. control. ^††p < 0.01; ^†††p < 0.001 vs. leptin.