Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 1;7:16710. doi: 10.1038/s41598-017-17165-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Subcellular localization of lnc-KDM5D-4:1 in HepG2 cells. (A,B) Cells without probes (negative control). (C,D) actin beta (ACTB fluorescence in situ hybridization (ACTB-FISH) (green) used as housekeeping gene and positive control for the RNA FISH assay. (F,G, and Supplementary Video S1) RNA FISH shows the nuclear localization of lnc-KDM5D-4:1 (red). Each probe was tested with (B,D,F,G, and Supplementary Video S1) or without (A,C,E) addition of 1% of acetic acid (AA) at the cell fixation step. This resulted in no observable nuclear signal for lnc-KDM5D-4:1 when AA is not added to the fixation solution (E). (H) Real-time PCR lnc-KDM5D-4:1 transcript expression results from nuclear and cytoplasmic RNAs isolated separately from HepG2 cells before reverse transcription, then real-time PCR. Results show a nuclear expression, exclusively, of lnc-KDM5D-4:1 in comparison to ACTB which is approximately half nuclear and half cytoplasmic. Microscopy confocal images represent three independent experiments (n = 3). Confocal microscope magnification: 60x.