Table 2.
Biophysical properties assessed for H and M LCs.
| LC | Source | Tmapp Far UV (°C) | Tmapp Near UV (°C)1 | ONSET ANS SIGNAL (°C) | Hydrophobicity (%) | Proteolysis2 | Trypsin sites |
|---|---|---|---|---|---|---|---|
| H3 | Rec | (40.2) – 54.4 ± 0.8 | (44.5)—53.1 | 54.8 | 23.1 | +++ | 14 |
| H6 | Rec | (43.9) – 54.5 ± 0.8 | (45.3)— | 55.3 | 23.1 | +++ | 18 |
| H7 | Rec | (42.8) – 54.7 ± 0.5 | (48.7) – 56.2 | 54.8 | 23.6 | ++ | 16 |
| H9 | BJP | 52.0 ± 0.8 | 54.0 | 55.8 | 21.3 | +++ | 14 |
| H10 | BJP | 54.9 ± 0.5 | 54.3 | 58.3 | 23.7 | ++ | 14 |
| H15 | BJP | 55.6 ± 0.6 | — | 54.7 | 21.8 | + + | 14 |
| H16 | BJP | 51.3 ± 0.7 | 51.9 | 54.5 | 22.3 | +++ | 13 |
| H18 | BJP | 52.4 ± 0.5 | 51.1 | 51.5 | 22.9 | ++ | 13 |
| M2 | BJP | 61.5 ± 0.8 | — | 63.9 | 23.0 | + | 15 |
| M7 | BJP | 54.7 ± 0.5 | 55.3 | 58.8 | 22.4 | + | 15 |
| M8 | BJP | 72.6 | — | 73.5 | 22.7 | + | 15 |
| M9 | Rec/BJP | (63.2) – 68.2 ± 1.4 | (58.8) – 67.7 | 62.8 | 24.2 | + | 15 |
| M10 | BJP | 58.0 ± 0.6 | — | 56.1 | 21.5 | + | 15 |
Rec, recombinantly expressed LC; BJP, Bence Jones proteins purified from urines. (1): some Tmapp values could not be measured due to protein aggregation during the temperature ramp. (2): +++: < 20%, ++: 20–60%, +: > 60% fraction of uncleaved protein after 180 min.