Table 4.
Identification of Borrelia proteins by Ge-LC-MS/MS in mouse skin infected with one of three different species of Borrelia burgdorferi sensu lato.
| Mouse ID # | B. burgdorferi ss N40 | B. afzelii NE4049 | B. garinii PBi | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 1 | 2 | 3 | |
| Spirochete density fla/104 gapdh | 24 | 10 | 43 | 304 | 151 | 3 | 69 | 92 | 38 | 112 | 14 | 30 | 19 |
| Mouse proteins | 3302 | 3458 | 3482 | 3440 | 3147 | 3580 | 3513 | 3009 | 2979 | 3077 | 3381 | 3374 | 3279 |
| Flagellin | X (4) | X (3) | |||||||||||
| VlsE | X (3) | X (1) | X (1) | X (1) | |||||||||
| GAPDH | X (2) | ||||||||||||
Mice were inoculated intradermally and the Borrelia infection was reactivated with clobetasol at 40 days post-infection. Proteins were identified using both Mascot and OMSSA algorithms. The numbers of identified peptides are in brackets.